Densitometry workbench
Blot lane densitometry
Drop in a blot image, adjust the lane boundaries by dragging, and read off integrated band intensities. Add a loading-control image to normalize. Everything runs in your browser — no image is uploaded.
Target blot
Drop image, or click to browse
PNG, TIFF, JPEG. Dark bands on light film.
Loading control optional
Drop control image
Same lane geometry — β-actin, GAPDH, tubulin. Used to normalize.
Measurement settings
Re-detects boundaries at profile minima.
About 2× band height. Larger = flatter background.
Rolling ball handles uneven film.
Relative values divide by this lane.
Drag the red boundaries to resize lanes, or the dashed blue lines to set the measured row band. Click a lane or table row to select it. The profile below shares the image's x-scale, so boundaries should sit in its valleys. Equal widths gives every lane an identical ROI area — best when bands are evenly spaced and you want areas directly comparable; it can leave signal outside the boxes, so check the profile afterwards.
Results
Load a blot image above to begin.
Lane boundaries are detected automatically, then you can adjust them by hand.